reference mouse anti bmp9 ab  (R&D Systems)

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: ( A–D ) ELISAs were performed to measure IgG2a ( A , C ) and anti-BMP9 Ab ( B , D ) levels in the serum of P6 neonates treated at P3 with vehicle (PBS), isotype control IgGs (IgG2a/2b), or anti-BMP9/10 Abs. Neonates were treated during lactation either from dams injected i.p. with ( A , B ), or by direct i.p. injections of ( C , D ), the different Abs or vehicle. Data represent mean ± s.e.m. (n = 5–7 pups per group from 2 dams); **** P < 0.0001, ANOVA.

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: Control, Injection

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: ( A – C ”) Representative images of fluorescent isolectin B4-stained retinas either from P6 neonates fed for 3 days by dams injected on P3 with PBS ( A ), control IgG2a/b Abs ( B ), or BMP9/10 blocking Abs ( C – C ”). ( C’ , C” ) are higher magnification images of the relevant boxed areas in C. a, artery; v, vein; scale bars, 500 μm. ( D–L ) Higher magnification showing retinal vasculature fields between an artery and a vein (Plexus, D–F ), or at the front of an artery ( G–I ) or a vein ( J–L ) from neonates treated as in ( A–C ). Scale bars, 100 μm. ( M–O ) Scatter plots showing the vascular density on retinal ‘petals’ at the plexus ( M ), artery front ( N ), or vein front ( O ). Data represent mean ± s.e.m. (n = 6 pups per group from 2 dams); **** P < 0.0001, ANOVA and Kruskal-Wallis test. Arrows indicate AVMs, defined as direct shunts between an artery and a vein.

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: Staining, Injection, Control, Blocking Assay

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: ( A,B ) Representative images of blue latex-perfused retinal vasculature of P6 neonates fed for 3 days by dams injected on P3 with control IgG2a/b Abs ( A ) or BMP9/10 blocking Abs ( B ). Arrows in B indicate AVMs. Scale bars, 500 μm. ( C ) Scheme depicting the method employed for the quantification of the number of latex dye-positive vessels. ( D ) Histogram showing the total number of vascular crosses. ( E ) Histogram showing the number of vascular crosses per concentric circle on circles 1 to 3. Data represent mean ± s.e.m. (n = 5–7 pups per group from 2 dams); **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05; ANOVA and Kruskal-Wallis test ( D , E ).

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: Injection, Control, Blocking Assay

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: ( A ) RNA-Seq heat map displaying differently expressed genes in mouse whole retinas following transmammary transfer of anti-BMP9/10 or control IgG2a/2b Abs (n = 6 pups per group from 1 dam). ( B ) HUVECs were treated or not (Ctrl) with ALK1-Fc (1 μg/mL, 24 h). Cell extracts were then analyzed by WB using Abs directed against the indicated proteins. ( C ) Densitometric analyses and quantification of phospho-Smad1/5/8, ID1, and ANG2 relative levels in three independent experiments as in ( B ). ( D ) ECs isolated from retinas of pups fed for 3 days by dams injected on P3 with control IgG2a/b Abs (Ctrl) or BMP9/10 blocking Abs (anti-BMP9/10) were analyzed for Id1 and Angpt2 mRNA levels by RT-qPCR. The results are expressed as relative levels of the control condition (n = 3 determinations, n = 6 pups per group from 1 dam). Data in ( C , D ) are mean ± s.e.m.; *** P < 0.001, ** P < 0.01, * P < 0.05, unpaired Student’s t-test.

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: RNA Sequencing, Control, Isolation, Injection, Blocking Assay, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: Top 25 retinal genes sorted by P -value, following transmammary-delivered immunoblocking of BMP9 and BMP10.

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: Sterility, Binding Assay, Sequencing